Dear all,
I’m not used to work with RNA. Recently, I made a RNA extraction using Qiaqene kit (QuiaShredder plus RNeasy Mini Spin). The 260/280 and 260/230 ratios are good (2.08 and 2.24) and I obtained 924.5ng/ul from 6x10e6 cells (aprox).
The problem is that running the gel in TBE in non-denaturing conditions I see 3 bands (2 main bands and a lighter 3dr bigger band, not two, as I expect. Some lab mates, with the same kit, have the same pattern.
Is this RNA Ok? Does anyone know why I have 3 bands?
Thank you very much!

Sometimes with a non-denaturing electrophoresis you see alternative RNA conformations. If you run the gel under denaturing conditions do you only see the 2 rRNA bands?

I think I must to try it...
Thank you very much, indeed!