I've selected the phage display antibody(scFv) library and transduced the phagemid into HB2151 for making scFv. But I can't get the scFv in several conditions, does any one know the optimal expression condition(especially the expression system,the concentration of glucose and IPTG, the tempreture,ect)which was determined empirically?
Thanks in advance!

Here is a protocol that may help:

http://www.nature.com/nprot/journal/v2/n12/full/nprot.2007.455.html

Also, I'm going to move this question to the antibody forum to see if someone else can also help.

Omai

Thank you very much!