Hello there
I have been trying to culture CHUB S7 but unsuccessfully.
The cells shrink and die after sometime of normal growth.
My colleagues asking me not to work with CHUB S7 becuase they are unreaiable and hard to maintain.
Is this ture ?
Is there anyone who has a successful experience with culturing CHUB S7 ?
Please help ??

PLease see the following information :
"Differentiation of Chub-S7 cells

Cells were grown in a mixture of DMEM/F12 media supplemented with 10% FBS, 2 mM glutamine, 100 U/ml penicillin/streptamycin until confluency. For differentiation, cells were cultured with a serum-free medium consisting of a mixture of DMEM/F12 media supplemented with 15 mM NaHCO3, 17 muM D-panthotenic acid, 15 mM Hepes, 33 muM biotin, 10 mug/ml transferrin, 1 nM triiodothyronin, 850 nM insulin and 500 mug/ml fetuin. This medium, referred to as 'basal medium', was supplemented with 1 muM Dex and 500 muM IBMX from day 0 to 3. Dex was maintained alone or with 1 muM BRL49653 from day 3 to 17 in order to promote differentiation. As stated in Results, OA complexed with bovine serum albumin (BSA; ratio OA-to-BSA: 5 : 1) was added to the medium containing BRL49653 from days 10 to 17. Lipid accumulation in differentiated cells was visualized by Oil-red O staining as previously described.13 All the experiments on Chub-S7 cell characterization were performed between passages 18 and 32 (69–122 PDs)."

This was published in Cell Death and Differentiation (2003) 10, 1025–1031. doi:10.1038/sj.cdd.4401273 by Darimont et al.

Dear Saswati1
thank you for your help
greatly appreciated.