How do you microdialyze a protein (antibody)? |
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Topic Started by Chemgod1
on 8/7/2008 11:46 AM
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Hi I have this antibody that I need to run on gel. But it's been purified under high salt conditions (0.5M NaCl). I know that the gel will give me problems if I run it under the high salt. However, I have been told I can microdialyze my protein. but how??
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Replies
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Yes, I've used the Slidealyzers too - they are excellent for small volumes. If you have larger volumes you might want to use a de-salting column. I used to use PD-10 columns for de-salting Adenovirus preps - there should be something available from ye olde Pharmacia (GE Healthcare now - I think).
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