Scientist Solutions: Life Science Discussions
 Refer A Friend    Link To Us    Bookmark Us       

English - Chinese             

Welcome to Scientist Solutions!

You are in General Forum >> Student Questions Sub-forum

URGENT: composition of Hepes Buffered saline

RSS Feed

Amritha Nair
India

Send PM
See Mini bio

Status: Tadpole
Tadpole



Topic Started by Amritha Nair
on 8/6/2008 9:59 AM
Reply to this post Go to the top of the page
what is the exact composition of the Hepes Buffered saline reagent??

Replies
amaranna6
India

Send PM
See Mini bio

Status: Tadpole
Frog Laureate



Posted By amaranna6 on 8/7/2008 0:40 AM
Reply to this post Go to the top of the page
Hi,

Please note the composition of HEPES saline,

HEPES 10mM;
NaCl 151mM;
KCl 4.7mM;
CaCl2 2mM;
MgCl2 1.2mM;
glucose 7.8mM.

The pH of this solution should be adjusted to 7.4 using NaOH.

AMAR
Helping hands are better than praying lips...


mchinmoyee
India

Send PM
See Mini bio

Status: Frog Laureate
Frog Laureate



Posted By mchinmoyee on 10/24/2008 1:57 AM
Reply to this post Go to the top of the page
Kindly follow the link also.

http://www.mpbio.com/product_info.php?products_id=15884

Importantly keep in mind phototoxicity of HEPES when exposed to ambient light due to the production of hydrogen peroxide. So its strongly advised to keep any HEPES containing solution in dark.

see theses refs :
* Toxicity of light-exposed Hepes media. Lepe-Zuniga JL, Zigler JS Jr, Gery I. Journal of Immunological Methods. 1987 Oct 23;103(1):145.
* Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium.
* Zigler JS Jr, Lepe-Zuniga JL, Vistica B, Gery I., In Vitro Cell Dev Biol. 1985 May;21(5):282-7.


Last edited Oct 23, 2008, 14:31 PM by mchinmoyee