dear all,

In the Nature methods of July 2008, J.S present the last applications
of the next-generation sequencing devices ( Applied Biosystems SOLid, 454 Roche, Illumina-solexa ).
Both sequencing platforms offer approximately similar read lengths, error rates and per-tag costs, but the distinction is specifically around the shotgun construction and the data analysis.
So I would like to discuss with the users of the Solexa method to know their feeling , about the device , the protocols, the limit of Solexa especially in the gene expression topic.
Thanks for your reply.
regards.

This paper may be useful for anyone considering completely ditching microarrays in favor of RNA-seq/WTSS. However, the dynamic range gain and sensitivity demonstrated in this one would sway me in favor of sequencing over arrays.

Ryan

The cost and turnaround time for RNA-seq vs microarrays may also be important factors. I am guessing microarrays are still (and will remain) faster and much cheaper than performing a similar experiment using RNA-seq.