Hi everybody !!!!!!!!!
This topic very interesting by performing based.
Also will be useful for scientist working with recombinant protein. This is a pharmacodynamics type of assay where we have to measure any dynamic activity of animals.
With this introduction I am submitting my problem.
I am trying to validation of bioassay in female balb/c mice, what I said in above. I am doing on assay with single injection of standard solution EPO, remain things are similar to Ph EU. monogram, but I am found large variation of retic count in inter group, so I am always failing in fudicial limit while calculating assay. As per Ph. Eu. other parameter like regression, linearity and parallelism are found ok but regression value found very low that is 7-15 only.
So, My question are
1) Is animal play role for failing of fudicial limit? how and why?
2) How I control fudicial limit?

Please try to guide me in these era
please join in this discussion
Ramesh

Is the size (weight) of the mice accounted for? Is there an age range or sex of the mice specified for the assay? Among these variables, I think the size of the mouse is most important. Older male mice can vary a lot in size within a cage and that could affect the variance in your data.

We have try to manage/control age and weight also. 6-8 week old and 2 gms SD for weight. Although we are getting such variations