Scientist Solutions - Glucose-free medium

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Glucose-free medium

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Noemie

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hello,

I'm using Iscove's modified dulbecco's medium in cell culture and I like to study my cell with and without glucose...So does anyone know - or have tryed -a glucose-free medium in cell culture?

Thks

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Posted Jul 18, 2008, 6:50 AM

guy

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Hi there,
I had a friend couple of years ago who used to use
Glucose-free McCoy’s 5A Medium. He did some thing with Human Brest carcinoma cells if I can recall.
I checked and found that Sigma got couple of different McCoy’s 5A Mediums.
Guy

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Posted Jul 18, 2008, 9:43 AM

samm

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said:

Most of the big companies offer glucose free variants of popular media, or even glucose amt variants (high and low glucose DMEM and RPMI for instance). IMDM should also be available from them, either ready-to-use, or a dropout medium formulation that you can prepare.

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Posted Jul 21, 2008, 11:04 AM

Tiffy

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Hi
keep in mind that you need special fcs. Dialyzed fcs is required because of traces of glucose in the fcs
Regards
Tiffy

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Posted Jul 22, 2008, 1:06 AM

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