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Reverse transcription using RNA primers

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mirage

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I wanted to know which is the best Reverse transcriptase (AMV-RT, MMLV-RT or SS) to use RNA primers for making the cDNA. I have a standard RNA template though.

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Posted Jul 10, 2008, 20:19 PM

qinglongyanyuedao

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SS seems the best to me

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Posted Jul 11, 2008, 9:46 AM

parvoman

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said:

I believe that the MMLV RT is more sensitive and thus better for small amounts of starting RNA (Stratagene's cDNA kit uses it and claims that it works with as little as 1ng RNA). I've just received the kit today to test so I'll see next week.

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Posted Jul 11, 2008, 18:31 PM

mirage

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Yes, but was wondering if anyone has had experiences with specifically RNA primers for cDNA construction, and NOT DNA primers....thanks...

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Posted Jul 11, 2008, 13:37 PM

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Reverse transcription using RNA primers