Hello Friends,
I am planning to grow microbes on a hydroxyapatite disc. Further I want to recover the viable microbes after couple of days. Please could you suggest me as to how can i recover my sessile microbes so that i could count the number of viable microbes in my solution.
Also, any suggestions as to how can i go about measuring the viable microbes after microbiciday treatment will be greatly appreciated.
Thanking you in advance.
Sincerely,
P

Hi Praseet,

I don't have any expertise in that area, but here are a couple of publications that may have the protocol you are looking for.

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T4J-4233N49-4&_user=4423&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000059605&_version=1&_urlVersion=0&_userid=4423&md5=ffd38b8444d03f5edb495d46e90919df

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VPN-43DK5FW-4&_user=4423&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000059605&_version=1&_urlVersion=0&_userid=4423&md5=9e855c655a736a10dda84671123dd578

Here are the authors in case the link doesn't work:

Staphylococcus aureus accessory regulators: expression within biofilms and effect on adhesion

Jonathan Pratten, , a, Simon J. Fosterc, Pan F. Chanc, Michael Wilsona and Sean P. Nairb

Selection of oral microbial adhesion antagonists using biotinylated Streptococcus sanguis and a human mixed oral microflora

Y. H. Guan, , T. de Graaf1, D. L. Lath, S. M. Humphreys, I. Marlow and A. H. Brook

Omai