I want to boil my samples (containing Laemli sample buffer + b-mercaptoethanol) at 100 degrees Celsius for 5minutes. But the cap of the microtube pops open above 95degrees (and I lose my sample). Does anyone have alternative ideas or solutions? I heard of a cover that can be clamped down on your microtubes to prevent the lids from opening, but I can't find it and don't know what it's called. Does anyone know where I can get one?

Hi There,
Another idea that I have used is to seal the cap with parfilm.
Go to VWR web page and find it or just call your VWR representative.
Guy

Hi there,
Did you manage to solve the problem?
Guy

you do not have to biol your sds sample, 70-75oC for 10min will do

Hi everyone,

Thanks for all your suggetsions. In the end, I bought LidLocks for Boiling from Scientific Specialists Inc. They go over the lid of the microtube and lock it in place, allowing me to boil at a steady 100oC. They work great, are cheap, reusable and I would recommend them to anyone who had the same problem I had. The only humourous thing is that they're bright neon colours, which looks so funny on my heating block.

Thaks again for all your suggestions.

Great news,
We are happy that you are sharing the information with all of us.
Now we hope that your study will be successful and that we would see the end result as a link to your article.
Guy