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Band quantification by photoshop [View Printable]
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R Bishop
Group: Admin Posts: 304 Joined: Jan 17, 2006
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I cant remember how to quantitate bands on my Southern blot film using Adobe photoshop can anyone help?
Rb
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......................... "To those who would tear the world down: We will defeat you. To those who seek peace and security: We support you. And to all those who have wondered if America's beacon still burns as bright: Tonight, we proved once more that the true strength of our nation comes not from the might of our arms or the scale of our wealth, but from the enduring power of our ideals: democracy, liberty, opportunity and unyielding hope."
-- Barack Obama, Nov. 4, 2008
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| Posted Jun 11, 2008, 10:33 AM |
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parvoman
Group: Member Posts: 256 Joined: Jul 28, 2005
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I haven't had to do this for a long time but we used the software associated with the Molecular Dynamics PhosphoImager.
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| Posted Jun 16, 2008, 14:11 PM |
Last edited Jun 16, 2008, 6:13 AM by parvoman |
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R Bishop
Group: Admin Posts: 304 Joined: Jan 17, 2006
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Anybody else out there know anything?
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......................... "To those who would tear the world down: We will defeat you. To those who seek peace and security: We support you. And to all those who have wondered if America's beacon still burns as bright: Tonight, we proved once more that the true strength of our nation comes not from the might of our arms or the scale of our wealth, but from the enduring power of our ideals: democracy, liberty, opportunity and unyielding hope."
-- Barack Obama, Nov. 4, 2008
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| Posted Jul 02, 2008, 17:57 PM |
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R Bishop
Group: Admin Posts: 304 Joined: Jan 17, 2006
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Nice this website really describes the method well. Also how to quantitate multiple blots
http://www.lukemiller.org/journal/2007/08/quantifying-western-blots-without.html
Rb
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......................... "To those who would tear the world down: We will defeat you. To those who seek peace and security: We support you. And to all those who have wondered if America's beacon still burns as bright: Tonight, we proved once more that the true strength of our nation comes not from the might of our arms or the scale of our wealth, but from the enduring power of our ideals: democracy, liberty, opportunity and unyielding hope."
-- Barack Obama, Nov. 4, 2008
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| Posted Jul 02, 2008, 18:07 PM |
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