Does anyone know of an electroporation device that uses the smallest volume for transfecting mammalian cells?

The reagents i am transfecting are extremely expensive and the smallest volume so that i can get the optimal local concentration for transfection is what i am looking for.

I am trailing the amaxa nuleofector at the moment, but the lowest volume that I have succeeded with is 50ul and it is very particular about its cuvettes.

I have another device that delivers 5ul of solution onto about a 2mm square area in the center of a 3.5mm dish. It is very efficient but very few cells see the pulse.

If there is a cuvette out there that uses 5-10ul of solution and is suitable for mammalian cell-line transfection I'd love to know about it (and the device to power it of course!)

There is a new Korean machine: Microporator that transfects directly in a special pipette tip: www.microporator.com

The tips are 10l or 100l

Why don't you try SONOporation, the use of high frequency ultrasound (1 or 3 MHz) by use of microbubbles for improved and much more sensitive transfection.

Contact me for more information or literature list

frasermoss said:

Does anyone know of an electroporation device that uses the smallest volume for transfecting mammalian cells? The reagents i am transfecting are extremely expensive and the smallest volume so that i can get the optimal local concentration for transfection is what i am looking for. I am trailing the amaxa nuleofector at the moment, but the lowest volume that I have succeeded with is 50ul and it is very particular about its cuvettes. I have another device that delivers 5ul of solution onto about a 2mm square area in the center of a 3.5mm dish. It is very efficient but very few cells see the pulse. If there is a cuvette out there that uses 5-10ul of solution and is suitable for mammalian cell-line transfection I'd love to know about it (and the device to power it of course!)