Hi all,
I am new to this community of scientists(even though I am not the one), I read some of the conversations and found them helpful so Thank you all...
I have a problem in patching L6 skeletal muscle cells, I am trying to make giga seal, but I couldn't form a gigaseal on these cells. I tried different types of pipettes and few other tricks, like applying -ve and +ve voltage, but still I can not seal these cells. Is there any one here, who has experience with these cells before? And if anyone can tell me which specific pipettes are good to seal L6 cells then it would be very helpful...