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SNaPshot

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JayM

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Joined: May 12, 2008







 Send a personal messsage to JayM Reply with a quote from this post Go to the top of the page

Has anybody ever used the SNaPshot multiplex kit (Applied Biosystems) to multiplex SBE primers that differ by 6 - 8 bases? My trial gave me results in which some of the SNPs that had been identified using singleplexes were suppressed in a multiplex reaction. Only the green peaks were not misbehaving. Has anybody ever faced this problem, or does anybody know how to solve it?

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 Posted May 12, 2008, 8:28 AM
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