I want to clone PPAR-gamma together with GFP genes into my rat bone marrow stem cells to induce the lipofibrolastic differentiation.
Would you please which vector is more simple and reliable to my experiments? As to the protocols, I would recommend the "PCR based molecular cloning protocols"
http://www.lab-manual.com/lm_177.htmAny advice related to PCR based molecular cloning is welcome. Thank you in advance.