I am interested in finding a fast acting protein synthesis inhibitors for cell culture studies. I am familiar with some inhibitors (anisomycin, cycloheximide), but these are slow for uptake by cells. I want to use a fast-acting inhibitor for B cell culture that are treated with TLR ligands. The idea is to prevent any protein synthesis during the treatment. Does anyone have any suggestions? Thanks in advance!

Apparently inducing Hypoxia can cause fast acting and reversible inhibition of protein synthesis

RegulationReticulum Kinase PERK and Phosphorylation of the Translation Initiation Factor eIF2{alpha}

Here's an alternative suggestion:

Trichothecene

Here's a PNAS refernce

Mechanism of Inibition of Eukaryotic Proetin Synthesis by Trichothecene

More info

http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/212215.htm

http://www.ansci.cornell.edu/plants/toxicagents/trichothecene.html

http://jp.physoc.org/cgi/content/abstract/322/1/447

http://aac.asm.org/cgi/content/abstract/11/3/491