changed current kinetics |
|
Would you like to save this topic, event, protocol or job so you can find it again easily?
Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.
Available to members only. Please log in or register for your free account now.
|
|
Topic Started by ash2008
on 5/6/2008 7:01 AM
|
|
hi,
i use whole cell patch clamp technique, to understand the functional properties of P2X3 receptors of the trigeminal mice neurons. i use the 24 hours incubated cell cultures to do patch clamp. P2X3 receptors are known to show rapid activation and very fast desensitization upon agonist application. Since one month and a half, i am facing problems in getting fast desensitization kinetics of P2X3 current. I changed all the buffer solutions, agonist stock, perfusion system, culture medium. But i am not getting back to the original situation of better current kinetics. Can somebody give some advices?
thank you
Last edited May 06, 2008, 17:00 PM by ash2008
|
Replies
|
|
|
Posted By bo
on 2/1/2010 5:45 AM
|
|
i guess, you see P2X2/3 or P2X2 receptors, but not P2X3 ones. Try to use DRG neurons.
|
|
As a Scientist Solutions member, you are able to register a positive vote for any topic which you believe is useful and relevant to our board or any reply which you believe is especially well worded and helpful.
By participating in the voting, you will be helping to identify the best topics & replies on the board.
You may vote once for any one post, and you may not vote for your own posts.
A post (topic or reply) will earn one "thumbs up" icon for every 10 votes received (up to 3 thumbs up), and the person who made the post will also earn two bonus points.
learn more about member points.
|
Become a member & join our
community (It's easy & free)
Already a member? Please log in
|
Life Technologies
|
|
Life Technologies
|
|
Bio-Rad Laboratories
|
|
7/6/2009
|
|
7/6/2009
|
|
7/6/2009
|
|
9/2/2010
|
|
8/30/2010
|
|
8/30/2010
|
|
|