changed current kinetics |
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Topic Started by ash2008
on 5/6/2008 7:01 AM
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hi,
i use whole cell patch clamp technique, to understand the functional properties of P2X3 receptors of the trigeminal mice neurons. i use the 24 hours incubated cell cultures to do patch clamp. P2X3 receptors are known to show rapid activation and very fast desensitization upon agonist application. Since one month and a half, i am facing problems in getting fast desensitization kinetics of P2X3 current. I changed all the buffer solutions, agonist stock, perfusion system, culture medium. But i am not getting back to the original situation of better current kinetics. Can somebody give some advices? thank you
Last edited May 06, 2008, 17:00 PM by ash2008
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Replies
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Posted By bo
on 2/1/2010 5:45 AM
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i guess, you see P2X2/3 or P2X2 receptors, but not P2X3 ones. Try to use DRG neurons.
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