hi,
i use whole cell patch clamp technique, to understand the functional properties of P2X3 receptors of the trigeminal mice neurons. i use the 24 hours incubated cell cultures to do patch clamp. P2X3 receptors are known to show rapid activation and very fast desensitization upon agonist application. Since one month and a half, i am facing problems in getting fast desensitization kinetics of P2X3 current. I changed all the buffer solutions, agonist stock, perfusion system, culture medium. But i am not getting back to the original situation of better current kinetics. Can somebody give some advices?
thank you