Dear all kind scientists,

Could you all please help me.. In what concentration of FBS, one cells still can adhere? Im planning to treat my cells in serum free condition.. however im worry that my cells would not adhere to the plate before i treat. So i would like to use FBS as minimum as possible..

Thankx for your suggestion..

Hi,
Depending what type are your cells. HeLa needs 10%.
Some other i.e. BHK require 5%.
Guy

You might want to pretreat the dish with poly-D-lysine as an adherence factor. This may help your cells stick in serum free media. I pretreat a well for 15min. in 0.01% poly-L-lysine at room temp., remove the lysine and let the dish dry, then add 293T cells for transfection.

Omai