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Can I use a 72 bp primer??

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Asha

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 Send a personal messsage to Asha Reply with a quote from this post Go to the top of the page

I have to use a primer which is 72 bp in length. it contains with 42 pb overhang. The reverse primer is shorted in lenth. I ve heard problems regarding the use of long primers, so want to know if this primer is advisable.

The primer sequence is as follows:
5' CAT ATG CAC CAC CAC CAC CAC CAT CTA GTA CCA AGA GGA TCA GCC AAC ATC GCG GTG CAG CGA ATC AAG CGG 3'

Thank you in advancce!!

.........................

 Posted Apr 24, 2008, 21:12 PM
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