Am having problems looking for IL1 beta and TNF alpha message in Zebrafish. There are publications that describe IL1 primers that work but in our hands they don't.

Does anyone have a pair of primer sequences that work - or even better, info for Taqman primers and probe that we could order?

Many thanks

PM

P.S. Does anyone else get different sized beta actin PCR products from 3day embryos compared to adult fish cells?