Best Stuff for Mounting [View Printable]
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vanishing
Group: Member
Posts: 129
Joined: Apr 25, 2005
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Can anybody recommend the best
I have immunostained brain slices (40 um thick)
what antifade kit can you recommend for mounting
any kits, any recipes
I used prolong antifade.. |
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 Posted Apr 11, 2008, 13:38 PM |
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frasermoss
Group: Admin
Posts: 722
Joined: Feb 22, 2005
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Vectashield antifade mounting medium (Vector Laboratories) |
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"Opportunity is missed by most people because it is dressed in overalls and looks like work". Edison
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| Posted Apr 15, 2008, 12:41 PM |
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krihol
Group: Member
Posts: 45
Joined: Jan 04, 2007
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Depending on how you are going to visualize it different media might bee needed. For general microscopy Vectashield is great. However, there might be some trouble if you are using a confocal. If you get trouble you can try prolongGold (Invitrogen). I have used both and I think that ProlongGold is a better alternative, but I'm mainly using a confocal. |
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| Posted May 13, 2008, 22:28 PM |
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samm
Group: Moderators
Posts: 407
Joined: Mar 03, 2005
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| vanishing said: | Can anybody recommend the best
I have immunostained brain slices (40 um thick)
what antifade kit can you recommend for mounting
any kits, any recipes
I used prolong antifade.. |
Prolong Gold is great for confocal and "cements" (no nail polish!), so if you are using only tissues, its great (bad for filters/matrices etc that can have mistaken orientation). The bad bit is that its expensive. A cheaper alternative is the FluorSave reagent from Calbiochem. VectaShield liquid formulation (Vector Labs) works pretty well, but i was quite disappointed with the "cementing" version. |
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| Posted May 14, 2008, 14:56 PM |
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samm
Group: Moderators
Posts: 407
Joined: Mar 03, 2005
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| vanishing said: | Can anybody recommend the best
I have immunostained brain slices (40 um thick)
what antifade kit can you recommend for mounting
any kits, any recipes
I used prolong antifade.. |
Prolong Gold is great for confocal and "cements" (no nail polish!), so if you are using only tissues, its great (bad for filters/matrices etc that can have mistaken orientation). The bad bit is that its expensive. A cheaper alternative is the FluorSave reagent from Calbiochem. VectaShield liquid formulation (Vector Labs) works pretty well, but i was quite disappointed with the "cementing" version. |
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| Posted May 14, 2008, 14:57 PM |
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