Hi everyone!
Can someone provide a protocol "for dummies" for CTL generation? With some troubleshooting for the most sensitive points or important cytokines.
Thanks a lot!
Monica

try these:

http://www.natureprotocols.com/2006/09/26/generation_of_multivirusspecif.php

Journal of Immunological Methods
Volume 210, Issue 1, 15 December 1997, Pages 65-77
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2Y-3SV3NRM-6&_user=1010281&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=1010281&md5=2f19c79fa8c187b6b7df383352dac287

or

http://www.ingentaconnect.com/content/els/00221759/1997/00000210/00000001/art00177

We could detect finally some CTL activity but only with Cr release, after months of not results with IFNg Elisa:(
Thanks for help