Can any body help me out in MTT assay, XTT assay

Dr.Prashant said:

Can any body help me out in MTT assay, XTT assay

Dear,
what is your question exactly? We're using MTT assay to detect cell proliferation but it could be useful to detect cytotoxicity. Help me with your trouble.
Look forward hearings from you

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Dr.Prashant said:

Can any body help me out in MTT assay, XTT assay

Standard Protocols
MTT Assay

This procedure is for cells in 96 well plates, if larger plates are used then adjust volumes accordingly.

1 Make a solution of 5mg/ml MTT dissolved in PBS and filter sterilise.

5 hours before the end of the incubation add 20m l of MTT solution from step one to each well containing cells.
Incubate the plate at 37?C for 5 hours.
Remove media with needle and syringe.
Add 200m l of DMSO to each well and pipette up and down to dissolve crystals.
Put plate into the 37?C incubator for 5 minutes to dissolve air bubbles.
Transfer to plate reader and measure absorbance at 550nm.