Very interesting information. Might I ask another question, related to whether analysis of EC50 versus IC50 will give the same answer. For example, consider a drug that yields a response of from 0% to 100% - one can analyze EC50. Another way of looking at the same data stems from the fact that the output being measured decreases in the presence of the drug. So, in the absence of the drug, what one measures (the output) is 100%. With increasing drug, this decreases to a value of 0%. One can analyze IC50.
My question is as follows. Can one analyze by either approach, that is by analzing the response data (analysis by EC50) or by analyzing the values as they decrease from 100% to 0% (analysis by IC50)? Will the same answer be obtained by either approach. Thanks very much.