Hi,
I was wondering is it possible to quantify miRNAs by any other method besides realtime-PCR? Is Northern (DIG/biotin probes) quantative?

Thanks

You can use Northern blot if you use a radioactive labeled probe and a house keeping gene to normalize with. If there is a biotin standard non-radioactive method should work in theory, but you will loose in sensitivity.

Whatever method you are using, you will produce much more robust quantitative results if you prepare a standard curve based on a synthetic miRNA of the same sequence as the analyte strand.