Hi all,

I'm working with HBsAg protein and I need a protocol to remove the adsorbed alum in commercial vaccines to retrieve the HBsAg.

I have tried using tri-sodium citrate to bind the alum, but I get no intact protein in the supernatant.

Can anyone help?

Thank you

take a look at these references

J Chromatogr A 2005, 1095, 119-25: "Ion-exchange chromatography of hepatitis B virus surface antigen from a recombinant Chinese hamster ovary cell line"


Vaccine 1997, 15, 637-47: "Comparison between hepatitis B surface antigen (HBsAg) particles derived from mammalian cells (CHO) and yeast cells (Hansenula polymorpha): composition, structure and immunogenicity"