Scientist Solutions: Life Science Discussions
 Refer a Friend    Link To Us    Bookmark Us       

      
 » Home » Diagnostics (Molecular, Pathological) » Blood Test/Pathogens » determining PCR product of determined primers

Other Topics
11/8/2008 07:06 AM
RT-PCR Product
2/5/2008 07:45 AM
G6PD diagnosis
8/8/2007 11:54 PM
Measuring antioxidant enz ...
4/11/2007 11:10 AM
PCR Amplification OF HCV ...
12/1/2006 03:37 PM
hcv vaccine
11/28/2006 06:12 AM
PCR Machine
7/2/2006 01:25 AM
ASK FOR YOUR HELP, I CAN ...
5/18/2006 06:08 PM
How to conduct a hemolisy ...
4/27/2006 03:35 PM
HIV testing by MEIA
12/13/2005 08:16 PM
2D for serum
11/22/2005 03:31 PM
using real time PCR for p ...
7/21/2005 10:23 AM
Rodent Coombs Reagent
1/5/2005 07:25 PM
any reliable serological ...
Subscribet to topic
Add Reply  Add New Topic  Add New Poll
bottom of page RSS Feed 

Topic Feed

 

determining PCR product of determined primers

 [View Printable]
biotech.helmi

Frog Egg

See
Similar
Scientists





Group: Member
Posts: 2
Joined: Feb 19, 2008







 Send a personal messsage to biotech.helmi Reply with a quote from this post Go to the top of the page

I am working on MTRR gene and i have specific primers from some article but i do not know what is the size that should i obtain ?
for a successful reaction , really i have PCR products and relatively all of them have the same size but i would like what is the specific size that should produce from those primers that i have ?
please help me

.........................

 Posted Feb 20, 2008, 1:15 AM
vanishing

Frog Laureate

See
Similar
Scientists





Group: Member
Posts: 129
Joined: Apr 25, 2005







 Send a personal messsage to vanishing Reply with a quote from this post Go to the top of the page

you will have to look at/download the cDNA sequence of your gene from pubmed and do an alignment of the primers.

the length between the beginning of your upstream primer and your downstream primer will determine the size of the PCR product.

you can download vector NTI 10 from Invitrogen if you are an academic user.

https://catalog.invitrogen.com/index.cfm?fuseaction=userGroup.userGroupHome&CFID=37373091&CFTOKEN=f42a26459f7e979f-4455DED8-5056-AC31-DA9C22E57345EF8F

and pubmed you will have access to, since you have internet.

alternatively, you could do a BLAST search with the primer sequence on pubmed.

.........................

Posted Feb 23, 2008, 3:30 AM
vanishing

Frog Laureate

See
Similar
Scientists





Group: Member
Posts: 129
Joined: Apr 25, 2005







 Send a personal messsage to vanishing Reply with a quote from this post Go to the top of the page

and what is the PCR template you are using? is that cDNA or genomic DNA?

.........................

Posted Feb 23, 2008, 3:33 AM
biotech.helmi

Frog Egg

See
Similar
Scientists





Group: Member
Posts: 2
Joined: Feb 19, 2008







 Send a personal messsage to biotech.helmi Reply with a quote from this post Go to the top of the page

genomic DNA

.........................

Posted Feb 24, 2008, 7:55 AM
top of page Add Reply  Add New Topic  Add New Poll

Forum Jump