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Washing G-beads after pre elution [View Printable]
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guy
Group: Moderators
Posts: 264
Joined: Nov 28, 2005
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Hi all,
Before eluting proteins from G-beads I am using 50mM Ammonium Bicarbonate.
Some how I still get some background after running it on MassSpec.
Do any one of you got another method of washing the beads before the elution.
Guy
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| Posted Feb 07, 2008, 19:33 PM |
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guy
Group: Moderators
Posts: 264
Joined: Nov 28, 2005
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I managed to find a solution.
I wash the beads once with the lysis buffer before washing with Ammonium Bicarbonate. Nearly noo background.
Guy
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| Posted Feb 15, 2008, 18:02 PM |
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guy
Group: Moderators
Posts: 264
Joined: Nov 28, 2005
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Some time I still have problems with the mass spec.
Now I am just washing teice with Ammonium Carbonate solution and the results are good
Guy
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| Posted Mar 20, 2008, 1:49 AM |
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guy
Group: Moderators
Posts: 264
Joined: Nov 28, 2005
|
Some time I still have problems with the mass spec.
Now I am just washing teice with Ammonium Carbonate solution and the results are good
Guy
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.........................
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| Posted Mar 20, 2008, 1:49 AM |
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