Dear friends
I want to know about pichia pastoris.I don't have any information about pichia psatoris media ,growth condition ,yield ,...
If you know anything about it ,I will be appreciate if you write
me.

thanks
f2

Here's a paper with a p pastoris media recipe and protein purification protocol

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WPJ-4CJCVD1-1&_user=1010281&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050264&_version=1&_urlVersion=0&_userid=1010281&md5=1825b1527bd27b839cbc072bedcdf716#toc3

Google search "pichia pastoris"

there are dozens of site with information and papers with experimental protocols

In the protocols section on this very site there is a protocol for electroporation of Pichia Pastoris

See the link below

http://www.scientistsolutions.com/index.php?a=protocol&c=28&p=797

Also see the reply by Tony Rook in this section

http://www.scientistsolutions.com/t2554-fermentation.html