Could anybody explain the methodology to isolate Neutrophil from Mouse peripheral blood. That would be halpful for me, if anyone give me the reference also.

Try this: Zhong, Z., and L. Pirofski. 1998. Antifungal activity of a human antiglucuronoxylomannan antibody. Clin. Diagn. Lab. Immunol. 5:58-64

It is Ficoll-based, fairly simple.

Good luck.