Dear all , I screen the promoter of HTR1A gene about a family and found a Mutation site.Then I use Dual Lusifearse Assay to observe the diifference between Wile and Mutation site and the result is expected!!! Now I'm going to see is any different in vitro so I design probe for EMSA...I want to know :There is some difference between wile and Mutation type ...How can I do to identified the Protein ??? Tks a lot
Do a supershift assay with Ab specific to your protein of interest
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