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Western Blot question

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biogeek0428

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 Send a personal messsage to biogeek0428 Reply with a quote from this post Go to the top of the page

I have a question - can you run fish protein on a regular agarose gel and then complete a Western Blot? All of the protocols I have run into call for the SDS-PAGE which I do not have the equipment for.

ANY HELP WOULD BE GREATLY APPRECIATED!!

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 Posted Jan 14, 2008, 0:08 AM
a_wandering_1

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In general, one does not run proteins on an agarose gel. SDS-PAGE has a much better ability to resolve proteins. Do you have another lab that can help you out with SDS-PAGE equipment? I've never been in a place that doesn't have someone working routinely with proteins.

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Posted Jan 25, 2008, 15:23 PM Last edited Jun 26, 2008, 19:04 PM by marcia
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