Hi guys,

I'm a student looking for some advice on this theoretical problem.

Problem:

I want to express a mammalian membraneprotein (= a heterodimer) in the yeast S. cerevisiae.

I have already constructed an expressionvector and transformede my cells.

Now I want to identify possible "bottlenecks" in the expression - which methods would you use to answer this???
I'm just looking for a few simpel ones