hello,everybody.I am choosing antibody for IF.there is little that can be chosen from.My cell line is mouse fibroblast.I want to test the expression of alpha-smooth muscle actin.There is an mouse monoclonal antibody which suit me ( price and application).But will I be questioned when I publish it? OR is there any method to remove the nonspecific binding？There is also antibody which can apply in IHC ,but should it also works well with IF?
thanks

There is a technique known as indirect immunodetection that you can use for mouse monoclonals on mouse tissue

here are some references

Paper
http://www.jhc.org/cgi/reprint/42/11/1499.pdf


Protocol

http://www.ihcworld.com/_protocols/general_IHC/immuno_mom.htm

you are so kind ! thank you very much!