Hi everyone:) can anyone tell me about a good source of human tissue for siolation of macrophages. I have read articles about using bronchial lavage fluids or granulation tissue. Does anyone has protocols to these procedures or any suggestions for a rich source of macrophages?
Thank you

Hi! unless the patient is suffering from an acute airway infection, you'll mostly get alveolar macs in BAL - and those are quite different from say, peritoneal, spleen or BM macs. If you have access to BM, there are some reports that have differentiated cells out of there, though you will have about 15% DC contamination.

Hey thanks...but can you give me any links to some protocols?

You could just take blood and isolate the monocytes (on the basis of plastic adherance) After about 5-7 days in culture with donor serum they will be mostly macrophages.

You can check this paper:
http://mrw.interscience.wiley.com/emrw/9780471142737/cp/cpim/article/im0706b/current/abstract


Isolation and Purification of Human Intestinal Macrophages

The gastrointest