It was really a strange discovery.
When P. aeruginosa was treated with yeast fermented broth for prolonged time, most cells died but mutants survived. They were as 100 times long as wild type; they didin't swim. Some of them produced reddish brown pigment. I have transferred them on agar slants several times for more than 3 years but most of them didn't lose their phenotype properties.
I can share my mutants to collaborate a project inquirng on the giant cells.
PS: I attach microphoto of a singel mutant cell.

Dr. Taing Ok, Kagoshima University, Japan
drtaingok@yahoo.com

How do I see the photo you mentioned--didn't see an attachment?

jim

ps.
if you want send reply to jachm888@yahoo.com


thanks,
jim

[Got the email with the photomicrograph--really fascinating!

Were the yeast treatments that induced the mutants done in broth with double the typical concentrations of carbon and nitrogen source--so as to reduce cell wall lysis?

jim










quote=drtaing]It was really a strange discovery.
When P. aeruginosa was treated with yeast fermented broth for prolonged time, most cells died but mutants survived. They were as 100 times long as wild type; they didin't swim. Some of them produced reddish brown pigment. I have transferred them on agar slants several times for more than 3 years but most of them didn't lose their phenotype properties.
I can share my mutants to collaborate a project inquirng on the giant cells.
PS: I attach microphoto of a singel mutant cell.

Dr. Taing Ok, Kagoshima University, Japan
drtaingok@yahoo.com
[/quote]