Hi

I'm willing to measure the activity of phosphofructokinase enzyme activity and wondering if any one has the simplest assay that i can use please reply appreciate the help and the post of the papers if there any.

Here are some links to publications using a PFK spectrophotometric assay

Phosphofructokinase in the Liver Fluke Fasciola hepatica
Link: http://www.jbc.org/cgi/reprint/261/9/4346.pdf

Studies on Heart Phosphofructokinase
Link: http://www.jbc.org/cgi/reprint/241/7/1512

Studies on Heart Phosphofructokinase: Purification, Inhibition, and Activation
Link: http://www.jbc.org/cgi/reprint/238/7/2285