Hi everyone:) i am using magnetic beads with specific antibodies to seperate macrophages. After the macrophages have been separated by positive selection, How do i seperate the macrophages from the magnetic beads?

Hi there,
If you are using Dynabeads please see the attached protocol from Invitrogen.
https://www.invitrogen.com/content/sfs/manuals/114.41D%20Dynabeads%20Mouse%20pan%20B%20(rev006).pdf
Look at section 2.3
Guy

Hi again:) well i am using magnetic particles made by a Russian firm, but the principle is actually the same. I have gone thru the protocol, but it does not say anything how to separate the macrophages from the magnetic beads. Any ideas?

From What I anderstand in section 2.3.5

5. For positive isolation, discard the supernatant
and wash the bead-bound cells 3 times by resuspending
in PBS to the original sample volume,
and separate using a magnet.

After the reaction with the beads and using the magnet the beads that reqacts with the magnet will have the Macrophages coupled .
So I guess afte taking off the Magnet you can spin the tube in a low G as the beads are much more heavy from the cells you can take the sup after spining the beads down.
I guess that the sup will have the cells that you are looking for .
Guy

Hey thanks Guy, i will try that.