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In Vitro Angiogenesis Assay [View Printable]
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Seagull
Group: Member
Posts: 22
Joined: Aug 27, 2005
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Hello,
Does anyone have the experience on this tube formation assay in 96-well plate? I just started to try HUV-EC-C cells on the assay by Chemicon Kit (Cat# ECM625). It worked well at first time. But, when repeating later, it did't work. I don't know what happened even I did it exactly the same way. Is it mostly possible the cells?
Thanks. |
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 Posted Nov 04, 2007, 9:11 AM |
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jabba
Group: Member
Posts: 4
Joined: Jan 02, 2008
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It might the BME itself. Matrigel, Cultrex, etc. are notorious for their lack of consistency from batch to batch. There are numerous companies out there with different angiogenesis assays that you might want to try out. |
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| Posted Feb 14, 2008, 20:56 PM |
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Seagull
Group: Member
Posts: 22
Joined: Aug 27, 2005
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Thanks a lot. And, could you please recommend a good one?
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| Posted Mar 13, 2008, 20:12 PM |
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jabba
Group: Member
Posts: 4
Joined: Jan 02, 2008
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Glycosan BioSystems recently contacted me in regards to beta testing their anti-angiogenesis product. I didn't take them up on it, but they said their stuff is defined (so you wouldn't have consistenct issues). It is a beta product, but it's also free.
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| Posted Sep 02, 2008, 20:27 PM |
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jabba
Group: Member
Posts: 4
Joined: Jan 02, 2008
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Glycosan BioSystems recently contacted me in regards to beta testing their anti-angiogenesis product. I didn't take them up on it, but they said their stuff is defined (so you wouldn't have consistenct issues). It is a beta product, but it's also free.
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| Posted Sep 02, 2008, 20:27 PM |
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