I need to do double blot, can I have detail protocol
http://www.millipore.com/techpublications/tech1/tp001en00protocolsPlease go to this URL and download the pdf file there. The double blotting section is from page 34-35. It is too big to be attached here.Table of Contents (Please see pdf file for complete protocols)1. Protein Transfer Protocols1.1 Electrotransfer: Tank (Wet) Transfer1.2 Electrotransfer: Semi-dry Transfer1.3 Dot Blotting/Slot Blotting: Vacuum Filtration1.4 Spot Blotting: Manual Spotting1.5 Optimization of Blocking Reagents Using Spot Blotting Method1.6 Double Blotting to Eliminate NSB1.7 Membrane Drying Methods2. Protein Visualization Protocols2.1 Visualization by Transillumination2.2 Visualization by Reversible StainingPonceau-S RedCPTS (Copper Phthalocyanine Tetrasulfonic Acid)2.3 Visualization by Irreversible StainingCoomassie Brilliant Blue R DyeAmido Black3. Immunodetection Protocols3.1 Standard Immunodetection Method3.2 Rapid Immunodetection Method3.3 High Salt Wash to Remove Persistent Background4. Membrane Stripping Protocols4.1 Stripping by Heat and Detergent4.2 Stripping by Acidic pH5. Protein Digestion Protocol5.1 On-Membrane Protein Digest for Mass Spectrometry6. Blot Storage Protocol6.1 Preparation of Blots for Long-Term Storage
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