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 Double Blot [View Printable]
krishna P. Reddy

Frog Egg

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Joined: Oct 22, 2007







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I need to do double blot, can I have detail protocol
.........................

 Posted Oct 27, 2007, 3:21 AM
Tracy

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http://www.millipore.com/techpublications/tech1/tp001en00protocols

Please go to this URL and download the pdf file there. The double blotting section is from page 34-35. It is too big to be attached here.

Table of Contents (Please see pdf file for complete protocols)
1. Protein Transfer Protocols
1.1 Electrotransfer: Tank (Wet) Transfer
1.2 Electrotransfer: Semi-dry Transfer
1.3 Dot Blotting/Slot Blotting: Vacuum Filtration
1.4 Spot Blotting: Manual Spotting
1.5 Optimization of Blocking Reagents Using Spot Blotting Method
1.6 Double Blotting to Eliminate NSB
1.7 Membrane Drying Methods

2. Protein Visualization Protocols
2.1 Visualization by Transillumination
2.2 Visualization by Reversible Staining
Ponceau-S Red
CPTS (Copper Phthalocyanine Tetrasulfonic Acid)
2.3 Visualization by Irreversible Staining
Coomassie Brilliant Blue R Dye
Amido Black

3. Immunodetection Protocols
3.1 Standard Immunodetection Method
3.2 Rapid Immunodetection Method
3.3 High Salt Wash to Remove Persistent Background

4. Membrane Stripping Protocols
4.1 Stripping by Heat and Detergent
4.2 Stripping by Acidic pH

5. Protein Digestion Protocol
5.1 On-Membrane Protein Digest for Mass Spectrometry

6. Blot Storage Protocol
6.1 Preparation of Blots for Long-Term Storage
.........................

Posted Oct 28, 2007, 20:36 PM
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