i want to get neutrophils out of the peritoneum after an injection of 1ml 5% thioglycollate solution. I removed the cells with 5ml 30% sucrose 6h after the injection. The problem is, the cell number 6h after the injection is not different to the cell number of uninjected mice. So the inflammation did not work?
Has anyone experience with this thioglycollate model and can give me a tip?

http://jimmunol.org/cgi/content/abstract/141/6/2090

You can check this paper for the details of this method.

I don't have experience in this.