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Storing DNA [View Printable]
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Dilip
Group: Member Posts: 24 Joined: Jul 24, 2007
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Will anybody please let me know ways of storing DNA
I'm extracting DNA from animal tissues using a kit called DNAZole and a conventionala method using lyses buffers with protienase K The kit results in producing a pellate and is stored in TE buffer it reccamends H2O or 8mM NaOH What ever did that pellet doesnt get dissolved Will any body please help me with your experiences Thanks dilip
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......................... Dilip K Fernando
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| Posted Oct 13, 2007, 0:22 AM |
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cfish
Group: Moderators Posts: 354 Joined: Sep 21, 2006
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I usually dissolve DNA in TE buffer and store at 20C or 4C depending on my usage. You want to avoid excessive freeze-thaw of the sample. Reference I just came across discussing TE vs water for storage of DNA. http://www.dnagenotek.com/pdf_files/PDPR012_LongTermStorage.pdf
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| Posted Feb 07, 2008, 21:14 PM |
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cfish
Group: Moderators Posts: 354 Joined: Sep 21, 2006
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I usually dissolve DNA in TE buffer and store at 20C or 4C depending on my usage. You want to avoid excessive freeze-thaw of the sample. Reference I just came across discussing TE vs water for storage of DNA. http://www.dnagenotek.com/pdf_files/PDPR012_LongTermStorage.pdf
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| Posted Feb 07, 2008, 21:16 PM |
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parvoman
Group: Member Posts: 190 Joined: Jul 28, 2005
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My tips would be:
Once you've pelleted the DNA and washed with 70% ethanol, let it air dry - but only just long enough that the pellet turns from the easily visible white to transparent. Pellets that have airdried for too long tend to be more difficult to resuspend in TE or water.
I used to use TE all
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