I am going to start working with Matrigel as a medium to study the development of endothelial proliferation and its inhibition, is there any particular protocol recommended? any special preacutions to be taken?. I also have my doubts about which cell line to use, we have normally worked with HUVEC but due to the difficulty to obtain appropiate viability etc... we are considering using an ATCC cell line, however there are several described in th bibliography and we have our doubts. I would greatly appreciate any help

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