Im looking for a protocol for non reducong conditions western blot.
Ive found that I just must no use DTT nor B-Mercaptoethanol in my loading buffer, for a SDS Page. Thats all??

Thanks!!

you may run a denatured non-reducing gel with just SDS in your sample buffer.

you may also run a native gel with everything remain the same but no SDS and DTT, and no SDS in running buffer either.

you may also run a blue native gel.

for the native gels, I have never done western, I am not sure if they will work properly.

Absolutely. Western blots work just fine when you run the gel in non-reducing conditions. The main problem you may run into may be that some antibodies are made to reduced proteins recombinant bands etc and may not react with native proteins. You may have to try several different ones to get a good signal.

Rb