Im looking for a protocol for non reducong conditions western blot.
Ive found that I just must no use DTT nor B-Mercaptoethanol in my loading buffer, for a SDS Page. Thats all??

Thanks!!

you may run a denatured non-reducing gel with just SDS in your sample buffer.

you may also run a native gel with everything remain the same but no SDS and DTT, and no SDS in running buffer either.

you may also run a blue native gel.

for the native gels, I have never done western, I am not sure if they will work properly.