I am going to produce a population of transgenic mice using lentiviral transduction methods, using sperm mediated transgenesis where eGFP will be used as reporter. Only 10% population is expected to be transgenic producing eGFP and the rest will be negative. Kindly suggest me a easy method to screening the eGFP producing transgenic mice from mixed population of eGFP expressing and non expressing mice population.

Ashwinraut - perhaps this article would help. A group of scientists did a study on the cellular distribution of enhanced green flourescent protein (EGFP)in the retina. They used transgenic and nontransgenic litter mates - so the article may describe how they sorted the mixed population of eGFP expressing mice. The link below will take you to the Investigative Opthamology and Visual Science Article:

" Absence of Functional and Structural Abnormalities Associated with Expression of EGFP in the Retina"

http://www.iovs.org/cgi/content/abstract/45/1/15

Written by: May Nour,1 Alexander B. Quiambao,2 Muayyad R. AlUbaidi,1,2 and Muna I. Naash1,2
1From the Oklahoma Center for Neuroscience, and the 2Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma.

PURPOSE. The present study was undertaken to evaluate the effect of uniform EGFP expression on retinal morphology and function.

Hope this helps : )

Thank you for the reference. I had gone through the article but procedure described is too tedious and specifically mentioned for retina so of little help.
Ashwin Raut