Dear Scientists and colleagues,

Im looking for a protease (every host is welcome) which cleaves specific at the N-terminus of its protease cleavage site, resulting in no additional amino acid residue (aa) at the C-terminus of target after cleavage (P1` site is the first aa of cleavage site and P1 is final C- terminal aa of target protein(...P3P2P1/P1P2P3...)).
Has anyone an idea where such protease is available, or does anyone know a host (vegetable, viral, mammalian or bacterial origin) producing such protease with this feature?

Thanks for your collaboration and for your efforts,
Best, MK.

Hello, I have heard of Lys-N It was recently published in a paper Nature Methods May 2008 Vol 5 No 5 One of the Authors was Shabaz Mohammed.

As far as the availability of Lys-N I think you can only get it from Japan


Protea BioSciences www.proteabio.com carries a wide selection of different Proteases

Hope this helps,

Cam

Lys-N is also known as Armillaria mellea neutral proteinase or Peptidyl-Lys metalloendopeptidase from Grifola frondosa and can be obtained from Seikagaku Corp in Japan. They sell in the US through Cape Cod Associates.

Good luck

Rb