Hi, guys, I am just wondering how sensitive is PI or DAPI in displaying overproliferation (or G/S check point disregulation).
I have seen increased BrDU incorporation in the knock out cells vs the wild type cells. I also see decreased p21 expression in knock out cells. However, when I check the cell cycle status using either PI or DAPI, I did not see any difference for the percentage of the S and G2/M phase cells. Any ideas? Suggestions? I have seen some people using Hoechst and pyronin. Will these two dyes make a difference ?

Thanks a lot in advance.